Recombinant Exendin-4
Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53% sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucose-dependent insulin secretion, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying in vivo. It also promotes ß-cell proliferation and neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coli expression of a synthetic DNA squence encoding the 39 amino acid of Exendin-4.
Reference
1. Malhotra R, Singh L, Eng J, et al. 1992. Regul Pept, 41: 149-56
2. Neidigh JW, Fesinmeyer RM, Prickett KS, et al. 2001. Biochemistry, 40: 13188-200
3. Movassat J, Beattie GM, Lopez AD, et al. 2002. J Clin Endocrinol Metab, 87: 4775-81
4. Alarcon C, Wicksteed B, Rhodes CJ. 2006. Diabetologia, 49: 2920-9.
| Accession # | P26349 |
| Alternate Names | Exenatide |
| Source | Escherichia coli. |
| M.Wt | Approximately 4.2 kDa, a single non-glycosylated polypeptide chain containing 39 amino acids. |
| AA Sequence | HGEGTFTSDL SKQMEEEAVR LFIEWLKNGG PSSGAPPPS |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | 1. Regulates Glucose levels rapidly;2. Reduces Insulin resistance;3. Reduces Glucagon;4. Reduces HbA1c;5. Stimulates beta cell growth which stimulates insulin production. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 10 EU/mg of rExendin-4 as determined by LAL method.