Raleukin

Alternate Names

Anakinra, AMG-719

Source

Escherichia coli.

M.Wt

17257.6

Cas No.

143090-92-0

Appearance

Liquid

Formula

C₇₅₉H₁₁₈₆N₂₀₈O₂₃₂S₁₀

Cell experiment

Proliferation, migration, and invasion: After an IL-1β (10 ng/mL)stimulation period of 3h, administration of anakinra (1 µg/mL, 48 h) strongly diminished expression of these cytokines in GBM and PBMC. Anakinra Dampened IL-1β-Induced Inflammatory Gene Expression in both cell populations¹.

ChIP assay: MEF cells were grown on 100 mm plates incubated with 10 μg/mL anakinra for 4 hours. Cells were then recovered in PBS 1× with MgCl₂ 1 mM, exposed to 1% formaldehyde at room temperature for 15 minutes to induce DNA crosslinking, washed, and then lysed with RIPA buffer containing 150 mM NaCl, 50 mM Tris pH 8.0, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% PIC, and PMSF².

Animal experiment

C57BL6 WT mice were treated (i.p.) with 10 mg/kg anakinra reconstituted in sterile water daily for 6 consecutive days beginning the day of the infection².

Target

IL-1

Pathway

Immunology/Inflammation

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles
- 12 months from date of receipt, -20 to -70 °C as supplied

Shipping Condition

Dry ice

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.