Ponceau S (sodium salt)
Ponceau S (sodium salt, CAS No. 6226-79-5) is a stain that enables the rapid and reversible detection of proteins immobilized on various matrices, such as nitrocellulose and polyvinylidene fluoride (PVDF) membranes. It is commonly used to visualize protein bands and assess the efficiency of protein transfer during blotting procedures. Reversible staining with Ponceau S has also been validated as an alternative to actin probing for use as a loading control. Additionally, this stain can serve as a fiducial marker in mass spectrometric imaging.
References：
[1] Yonan CR, Duong PT, Chang FN. High-efficiency staining of proteins on different blot membranes. Anal Biochem. 2005 Mar 1;338(1):159-61. doi: 10.1016/j.ab.2004.11.010. PMID: 15707947.
[2] Romero-Calvo I, Ocón B, Martínez-Moya P, Suárez MD, Zarzuelo A, Martínez-Augustin O, de Medina FS. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem. 2010 Jun 15;401(2):318-20. doi: 10.1016/j.ab.2010.02.036. Epub 2010 Mar 3. PMID: 20206115.
[3] Chughtai K, Jiang L, Greenwood TR, Klinkert I, Amstalden van Hove ER, Heeren RM, Glunde K. Fiducial markers for combined 3-dimensional mass spectrometric and optical tissue imaging. Anal Chem. 2012 Feb 21;84(4):1817-23. doi: 10.1021/ac203373h. Epub 2012 Feb 7. PMID: 22283706; PMCID: PMC3302962.
| Storage | Store at RT |
| M.Wt | 760.57 |
| Cas No. | 6226-79-5 |
| Formula | C22H12N4Na4O13S4 |
| Synonyms | Acid Red 112 |
| Chemical Name | sodium 3-hydroxy-4-((E)-(2-sulfonato-4-((E)-(4-sulfonatophenyl)diazenyl)phenyl)diazenyl)naphthalene-2,7-disulfonate |
| SDF | Download SDF |
| Canonical SMILES | O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C(C=C(S(=O)(O[Na])=O)C=C4)=C1)(O[Na])=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
