NP-G2-044

NP-G2-044 (CAS No.: 1807454-59-6) is a synthetic small-molecule cytoskeleton modulator and oncology research tool compound designed for biomedical research and drug discovery workflows. It targets the actin-bundling protein fascin and inhibits fascin-mediated filamentous actin crosslinking, thereby functionally perturbing filopodia formation as well as adhesion dynamics and cytoskeletal remodeling programs associated with cell migration and invasion; given the key role of fascin in metastasis-associated phenotypes and immune cell migration, this compound can serve as a controllable intervention tool for dissecting related biological processes and network connections. In vitro studies show that its activity falls within the submicromolar to low micromolar range, with specific readouts depending on experimental conditions and cellular context, supporting its use in target validation, mechanism-of-action elucidation, and phenotypic screening; typical use scenarios include cell-based filopodia assembly and morphological assays, scratch wound healing and transwell migration/invasion assays, three-dimensional tumor spheroid or organoid invasion models, and characterization of metastasis-related behavior and tumor–immune interactions in animal studies. NP-G2-044 is suitable for exploring mechanisms of actin cytoskeleton regulation, metastasis biology, and immuno-oncology, and can be used in discovery-stage workflows for benchmarking fascin dependence or investigating combination strategies in controlled research models; the concentrations or doses used in experiments generally need to be optimized and determined according to the specific experimental design and research objectives, and all applications are limited to life science research purposes.