Myeloperoxidase Chlorination Fluorometric Assay Kit

Myeloperoxidase (MPO) is an important member of the heme peroxidase-cyclooxygenase superfamily, which is expressed in a variety of inflammatory cells such as neutrophils, activated microglia, monocytes, and is essential for immune surveillance and host defense. Studies have shown that MPO plays a key role in a variety of diseases and is a potential biomarker and therapeutic target for inflammatory and oxidative stress-related diseases.

MPO can catalyze two types of redox reactions, including chlorination and peroxidation. The Myeloperoxidase Chlorination Fluorometric Assay Kit provides a rapid, simple, and sensitive method for detecting myeloperoxidase (MPO) activity by chlorination. MPO can catalyze the production of hypochlorous acid (HClO). Subsequently, HClO can react with the fluorescent probe APF to form Fluorescein, which can be detected at excitation/emission wavelength = 485/525 nm and its fluorescence signal intensity is positively correlated with MPO activity. In addition, MPO activity can also be detected by the detection of MPO peroxidation, the Myeloperoxidase Peroxidation Fluorometric Assay Kit (Cat. No. K2285).

Figure 1: MPO catalytic mechanism

Figure 2: Fluorescein Standard Curve

Figure 3: Measurement of MPO activity in mouse serum (2-fold dilution)