Mayer's Hematoxylin Stain Solution (For IHC)

Hematoxylin is a basic natural dye that stains cell nuclei. The primary component of nuclear chromatin is DNA; within the double helix structure of DNA, phosphate groups on the two nucleotide chains face outward, causing the exterior of the DNA double helix to carry a negative charge and exhibit acidic properties. Consequently, it readily binds to the positively charged hematoxylin basic dye via ionic or hydrogen bonds, resulting in staining.

This solution is a progressive hematoxylin staining solution designed for counterstaining tissue sections in immunohistochemistry (IHC). Typically, hydrochloric acid ethanol differentiation is not required, and the stained cell nuclei appear blue. This product is a ready-to-use working solution. The staining solution can be reused multiple times; however, staining performance will gradually diminish.