Magnesium Colorimetric Assay Kit
Magnesium is the 11th most abundant element in the human body. Mg2+ is essential for all living cells where it is involved in facilitating the processing of biological polyphosphates like DNA, RNA, ATP and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll. Mg2+ can be used as antacids and laxatives, and used to stabilize blood vessel spasm such as eclampsia and abnormal nerve excitation.
The Magnesium Colorimetric Assay Kit provides a simple and sensitive way for detection of magnesium concentration in various biological samples based on colorimetric method and the specific requirement of glycerol kinase for Mg2+. The enzyme linked reaction generates an intensely colored (λmax = 450 nm) product whose formation is proportional to Mg2+ concentration. The colored product can be easily qualified using a microtiter plate reader or a spectrophotometer at 450 nm. The linear range is 2-15 nmoles with detection sensitivity ~ 40 µM.
| Features | Fast and convenient Simple procedure; takes less ~30 minutes The assay is sensitive, stable and high-throughput adaptable. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. |
| Shipping | Blue Ice |
| Storage Conditions | Store at +4°C. |
| Kit components | Magnesium Assay Buffer Magnesium Developer Magnesium Enzyme Mix Magnesium Standard (150 nmol/µL) |