L 012 sodium salt
L 012 sodium salt is a chemiluminescent probe [1].
L 012 sodium salt is a new chemiluminescent probe. In activated EoL-1 cells which generated reactive oxygen species (ROS), treatment with L-012 generated a significant chemiluminescence. While, superoxide dismutase (100-300 U/ml) abolished the chemiluminescence, which suggested that the main ROS detected was O2- [1]. In cultured endothelial cells, phorbol 12-myristate 13-acetate (PMA, 1 μM) caused an 2.8-fold increase of L-012 chemiluminescence. Vanadate (100 μM) increased the chemiluminescence up to 17-fold. These results suggested that the increase of superoxide produced by NAD(P)H oxidase [2].
In human oral cavity and blood and rat peritoneal cavity, L-012 reacted with ROS generated by activated neutrophils and generated strong chemiluminescence, which was higher than that of MCLA [3]. In nonobese diabetic mice and K8 knockout (K8(-/-)) mice, treatment with dextran sulphate sodium generated an increased L-012 chemiluminescence which detected reactive oxygen and nitrogen species (ROS and RNS) [4].
References:
[1]. Nishinaka Y, Aramaki Y, Yoshida H, et al. A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells. Biochem Biophys Res Commun, 1993, 193(2): 554-559.
[2]. Sohn HY, Gloe T, Keller M, et al. Sensitive superoxide detection in vascular cells by the new chemiluminescence dye L-012. J Vasc Res, 1999, 36(6): 456-464.
[3]. Imada I, Sato EF, Miyamoto M, et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012. Anal Biochem. 1999, 271(1): 53-58.
[4]. Asghar MN, Emani R, Alam C, et al. In vivo imaging of reactive oxygen and nitrogen species in murine colitis. Inflamm Bowel Dis, 2014, 20(8): 1435-1447.
Physical Appearance | Yellow solid |
Storage | Store at -20°C |
M.Wt | 310.67 |
Cas No. | 143556-24-5 |
Formula | C13H8CIN4NaO2 |
Solubility | <15.53mg/ml in H2O; <31.07mg/ml in DMSO |
Chemical Name | sodium 8-amino-5-chloro-4-hydroxy-7-phenylpyrido[3,4-d]pyridazin-1-olate |
SDF | Download SDF |
Canonical SMILES | ClC1=NC(C2=CC=CC=C2)=C(N)C3=C1C(O)=NN=C3[O-].[Na+] |
Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
