Toggle Nav
Close
  • Menu
  • Setting

L 012 sodium salt

Catalog No.
B5806
new chemiluminescent probe
Grouped product items
SizePriceStock Qty
5mg
$90.00
Ship with 5-10 days
10mg
$150.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

L 012 sodium salt is a chemiluminescent probe [1].

L 012 sodium salt is a new chemiluminescent probe. In activated EoL-1 cells which generated reactive oxygen species (ROS), treatment with L-012 generated a significant chemiluminescence. While, superoxide dismutase (100-300 U/ml) abolished the chemiluminescence, which suggested that the main ROS detected was O2- [1]. In cultured endothelial cells, phorbol 12-myristate 13-acetate (PMA, 1 μM) caused an 2.8-fold increase of L-012 chemiluminescence. Vanadate (100 μM) increased the chemiluminescence up to 17-fold. These results suggested that the increase of superoxide produced by NAD(P)H oxidase [2].

In human oral cavity and blood and rat peritoneal cavity, L-012 reacted with ROS generated by activated neutrophils and generated strong chemiluminescence, which was higher than that of MCLA [3]. In nonobese diabetic mice and K8 knockout (K8(-/-)) mice, treatment with dextran sulphate sodium generated an increased L-012 chemiluminescence which detected reactive oxygen and nitrogen species (ROS and RNS) [4].

References:
[1].  Nishinaka Y, Aramaki Y, Yoshida H, et al. A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells. Biochem Biophys Res Commun, 1993, 193(2): 554-559.
[2].  Sohn HY, Gloe T, Keller M, et al. Sensitive superoxide detection in vascular cells by the new chemiluminescence dye L-012. J Vasc Res, 1999, 36(6): 456-464.
[3].  Imada I, Sato EF, Miyamoto M, et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012. Anal Biochem. 1999, 271(1): 53-58.
[4].  Asghar MN, Emani R, Alam C, et al. In vivo imaging of reactive oxygen and nitrogen species in murine colitis. Inflamm Bowel Dis, 2014, 20(8): 1435-1447.

Chemical Properties

Physical AppearanceYellow solid
StorageStore at -20°C
M.Wt310.67
Cas No.143556-24-5
FormulaC13H8CIN4NaO2
Solubility<15.53mg/ml in H2O; <31.07mg/ml in DMSO
Chemical Namesodium 8-amino-5-chloro-4-hydroxy-7-phenylpyrido[3,4-d]pyridazin-1-olate
SDFDownload SDF
Canonical SMILESClC1=NC(C2=CC=CC=C2)=C(N)C3=C1C(O)=NN=C3[O-].[Na+]
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Quality Control & MSDS

View current batch:

Chemical structure

L 012 sodium salt