HyperTrap Phenyl HP Column
HyperChrom Phenyl HP Agarose is a class of hydrophobic interaction chromatography media that separates molecules using differences in their hydrophobic properties and how much they are under specific conditions. The HyperChrom Phenyl HP Agarose series has excellent scale-up performance: 1) Highly crosslinked agarose base frame with excellent rigidity, so high process flow rates and improved process efficiency can be achieved at low backpressure. 2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. 3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. 4) Fine particle size design to improve resolution.
HyperTrap Phenyl HP Column is a preloaded column with HyperChrom Phenyl HP Agoros as the chromatography medium, which is mainly used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, viral vaccines, etc.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap Phenyl HP Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap Phenyl HP Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Hydrophobic interactions |
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Ligation |
R-O-CH2-CH(OH)-CH2 -O-C6H5 |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
34 μm |
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Ligand density |
25 μmol phenyl group/mL chromatography medium |
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Dynamic load |
>45 mg α-chymotrypsinogen/mL chromatography medium* |
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Flow rates are recommended |
90-150 cm/h |
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Maximum flow rate |
200 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability** |
2-14 |
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Solvent resistant |
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol***, 75% ethanol***, 10% ethylene glycol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
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Intolerant to solvents |
Oxidant |
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*Dynamic load measurement conditions: column height: 10 cm, test flow rate 150 cm/h, test buffer: 0.1 M potassium phosphate, 2 M ammonium sulfate solution, pH 7.0, test sample: 2mg/m L of α-chymotrypsinogen sample, when the penetration of α-chymotrypsinogen reaches 10%, the amount of α-chymotrypsinogen per unit volume (mL) (mg). **After 7 days in the environment of chromatography at 40oC and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. *** 30%, 75% and 10% are v/v, volume ratio. |
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