HyperChrom SP HP Agarose
HyperChrom SP HP Agarose is a strong cation exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom SP HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base with excellent rigidity, so high process flow rates and improved process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. (3) Fine particle size design to improve resolution.
HyperChrom SP HP Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Strong cation exchange |
|
Ligation |
- (CH2)3SO3- (sulfopropyl). |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
34 μm |
|
Ion load |
0.15-0.20 mmol H+ /mL chromatography medium |
|
Dynamic load |
>50 mg β-lactoglobulin/mL chromatography medium* |
|
Flow rates are recommended |
90-150 cm/h |
|
Maximum flow rate |
200 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, cationic detergent |
|
*Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 150 cm/h, test buffer: 0.1 M Citrate solution, pH 8.0, test sample: 4 mg/m Lβ-lactoglobulin sample, when the penetration of β-lactoglobulin reaches 10%,β-lactoglobulin loading volume per unit media volume (mL) (mg). **After 7 days of storage of chromatography medium at 40°C and pH 2-14, its physicochemical properties and functions did not change significantly. ***30% and 75% are v/v, volume ratio. |
|
| Components | PC2015-25 mL | PC2015-100 mL |
| HyperChrom SP HP Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||