HyperChrom SP FF Agarosee
HyperChrom SP FF Agarose is a strong cation exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom SP FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom SP FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Strong cation exchange |
|
Ligation |
- (CH2)3SO3- (sulfopropyl). |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ion load |
0.18-0.25 mmoll H+/mL chromatography medium |
|
Dynamic load |
>50 mg β-lactoglobulin/mL chromatography medium* |
|
Flow rates are recommended |
300-600 cm/h |
|
Maximum flow rate |
700 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, cation detergent |
|
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.1 M Citrate solution, pH 3.0; test sample: 4 mg/ml β-lactoglobulin sample, when the penetration of β-lactoglobulin reaches 10%, the amount of β-lactoglobulin per unit volume of media (mL) (mg). **After 7 days in the environment of chromatography at 40°C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ***30% and 75% are v/v, volume ratio. |
|
| Components | PC2014-25 mL | PC2014-100 mL |
| HyperChrom SP FF Agarosee | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||