Human CD34 Cell Enrichment Kit (Negative Selection)
The Human CD34+ Cell Enrichment Kit (Negative Selection) is used for the isolation of CD34+ cells from human PBMCs or CBMCs by negative selection. The isolation principle of this kit is to label unwanted cells with the biotin-labeled monoclonal antibody mix, and then add streptavidin-labeled magnetic beads to remove unwanted cells. Isolated cells are high-purity CD34+ cells.
This kit is easy to use and does not require a separation column, which only requires a magnetic separator to isolate cells in as little as 30 min. Generally, one-step enrichment can achieve 10-fold concentration, and two-step enrichment can achieve 30-fold concentration. At the same time, the obtained cells are not labeled with antibodies and magnetic beads, so they will not be abnormally activated and will not affect downstream experiments.
Components |
100 Assays (for 109 cells) |
Storage |
Biotin-Antibody Mix |
200 μL |
4°C |
Streptavidin Beads |
2 mL |
4°C |
Shipping: Blue ice Shelf life: 1 year |