HDL and LDL/VLDL Cholesterol Assay Kit

Cholesterol in blood is mainly transported as lipoproteins, including HDL-C, LDL-C, and VLDL-C. HDL-C, rich in ApoA-I, facilitates reverse cholesterol transport and exhibits antioxidant and anti-inflammatory properties; reduced levels correlate with increased atherosclerotic cardiovascular disease (ASCVD) risk. LDL-C, containing ApoB-100, holds the highest cholesterol content in plasma and is a primary target for lipid intervention. VLDL-C, synthesized in the liver, transports endogenous triglycerides and converts to LDL-C; its elevation indicates metabolic disorders. Accurate measurement of HDL-C and LDL/VLDL-C is vital for disease and health management.

This kit utilizes chemical precipitation and enzymatic reactions for efficient lipoprotein separation and quantification. Following precipitation, cholesterol esters in HDL-C or LDL/VLDL-C fractions are hydrolyzed by cholesterol esterase and cholesterol oxidase to produce hydrogen peroxide, which reacts with Amplex Red via horseradish peroxidase to generate a fluorescent signal proportional to cholesterol content. Compatible with serum, plasma, and tissue samples, the kit offers high sensitivity and a wide linear range, supporting both fluorescence and absorbance detection for basic research and high-throughput screening.

Figure 1. Typical standard curve and assay data. (A) Reference standard curve obtained via colorimetric detection. (B) Reference standard curve obtained via fluorometric detection. (C) Measurement of HDL, LDL/VLDL, and Total cholesterol in various samples using this kit.