Gradient-like PAGE Gel Rapid Electrophoresis Kit
This product is a gradient-like PAGE gel preparation kit. Through a unique premixed formulation for the upper and lower gel layers, it enables broad-spectrum separation capability (10–250 kDa) similar to gradient gels under simplified operation. It allows low-, medium-, and high-molecular-weight proteins to be evenly distributed on a single gel, eliminating the need to select different lower-gel concentrations based on the target protein molecular weight. The kit contains all the components required for gel casting, along with a dedicated electrophoresis buffer powder—ready to use, convenient, and highly efficient.
The product features the following characteristics: ① Adopts a one-step gel casting design—after pouring the lower gel, there is no need to wait for it to polymerize before directly adding the upper gel, eliminating the traditional liquid-sealing step; ② The upper gel is blue, making the loading wells clearly visible and significantly improving the sample-loading experience; ③ Equipped with an improved accelerator that offers good stability and high catalytic efficiency—no additional TEMED is required during gel preparation, completely eliminating the unpleasant odor associated with traditional gel casting; ④ Comes with a dedicated electrophoresis buffer (powder), allowing electrophoresis to be completed in approximately 25 minutes under constant voltage at 200 V, greatly shortening the experimental cycle.
Each kit can prepare approximately 50 mini-gels (0.75 mm thickness). The reagents are valid for 12 months when stored at 2–8°C in the dark, and the accompanying electrophoresis buffer powder can be stored at room temperature for up to 2 years.
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Components |
50 T |
Storage |
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Reagent A (upper glue liquor, 2X) |
40 mL |
4°C away from light |
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Reagent B (upper glue buffer, 2X) |
40 mL |
4°C |
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Reagent C (lower glue liquor, 2X) |
120 mL |
4°C away from light |
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Reagent D (lower glue buffer, 2X) |
120 mL |
4°C |
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Reagent E (PAGE Adhesives) |
5 mL |
-20°C away from light |
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Reagent F (Gradient-Like PAGE Running Buffer, Powder) |
10× 500 mL |
Room temperature |
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Shipping: Blue ice |
Shelf life: 12 months |
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One-step casting: Pour resolving gel, add stacking gel directly – no sealing, no waiting.
Colored stacking gel: Easy loading and gel identification.
Quick prep: No calculations or dilutions – cast multiple gels rapidly.
Broad range: 10–250 kDa – one gel for all.
Fast run: ~25 min at 200 V.
Odor-free: No TEMED, no bad smell.
