Glucose Colorimetric Assay Kit (O-toluidine Method)

Glucose is a key energy metabolism molecule in living organisms, widely involved in physiological processes such as cellular respiration and glycogen synthesis, and has important clinical significance in the diagnosis and research of diseases such as diabetes and metabolic syndrome. Glucose concentration detection is essential for clinical testing, biological research, and drug development.

This kit is based on the O-toluidine colorimetric method, where glucose reacts with o-toluidine under acidic conditions to form a blue-green product. The absorbance of this product is directly proportional to the glucose concentration and can be quantitatively measured at 630 nm. This kit is easy to use and highly sensitive, and suitable for various sample types such as serum, plasma, and cells. Compared with another common glucose detection method (GOD-POD method), the O-toluidine method only develops color with aldose and is not affected by other reducing substances, making it very suitable for the detection of glucose in serum, urine and other solutions.

Figure 1: Glucose standard curve for this kit. Figures A and B show the curve using 5 μL standard, and Figures C and D show the curve using 20 μL standard.