Glucose Colorimetric Assay Kit (GOD-POD Method)

Glucose is a key energy metabolism molecule in living organisms, widely involved in physiological processes such as cellular respiration and glycogen synthesis, and has important clinical significance in the diagnosis and research of diseases such as diabetes and metabolic syndrome. Glucose concentration detection is essential for clinical testing, biological research, and drug development.

Glucose oxidase (GOD) can catalyze the oxidation of glucose to D-gluconic acid and H₂O₂. H₂O₂ can react with phenol and 4-aminoantipyrine under the action of peroxidase (POD) to form quinonimine, a red product. The absorbance of quinonimine at 505 nm is directly proportional to the glucose concentration. The kit is easy to use and highly sensitive, and is suitable for a variety of sample types such as serum, plasma, and tissue. This kit is more sensitive than another common glucose kit (O-toluidine method).

Figure 1: Glucose standard curve for this kit