EZ Cap™ ZsGreen mRNA
ZsGreen is currently one of the brightest green fluorescent proteins, originally derived from the coral Zoanthus sp., and exists as a tetramer in its native state. Compared with traditional GFP and its common variants, ZsGreen exhibits superior spectral properties and much stronger fluorescence, with a brighter green color. Its excitation and emission wavelengths are approximately 493 nm and 505 nm, respectively, and its brightness can reach several times that of enhanced green fluorescent protein (EGFP). In applications such as cell labeling and protein localization, ZsGreen can generate clearly distinguishable fluorescent signals even at low expression levels, greatly facilitating detection and imaging.
Key features:
Cap1 Structure: The mRNA incorporates a Cap1 analog at the 5' end, ensuring high translation initiation efficiency, improved stability, and reduced innate immune recognition, leading to stronger and more sustained luciferase expression.
poly(A) tail: It contains an optimized poly(A) tail of approximately 100 nucleotides. This specific length is engineered to maximize transcript stability by resisting degradation and to synergize with the 5' cap for superior, sustained translation efficiency, ensuring robust protein yield in your applications.
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mRNA Length |
952 nucleotides |
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Concentration |
~1 mg/mL |
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Excitation max (nm) |
493 nm (ZsGreen) |
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Emission max (nm) |
505 nm (ZsGreen) |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
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General tips |
Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. |
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Shipping Condition |
ship with dry ice |
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Related Biological Data
