EZ Cap™ SaCas9 mRNA (m1Ψ)
SaCas9 is a CRISPR nuclease derived from Staphylococcus aureus. It is smaller than the canonical SpCas9 from Streptococcus pyogenes, and facilitates efficient vector packaging. Guided by sgRNA, SaCas9 recognizes target DNA and the PAM sequence 5'-NNGRRT-3', and cleaves double-stranded DNA via its HNH and RuvC domains. This activates cellular NHEJ or HDR repair pathways to achieve gene knockout, insertion, or correction.
SaCas9 recognizes a longer PAM motif and exhibits lower off-target risk compared with SpCas9. It is widely applied in research gene editing, disease model construction, and preclinical gene therapy development.
Key features:
Cap1 Structure: The mRNA incorporates a Cap1 analog at the 5' end, ensuring high translation initiation efficiency, improved stability, and reduced innate immune recognition, leading to stronger and more sustained luciferase expression.
Modified Nucleotides: It contains N1-Methylpseudo-UTP modified nucleotides, which decreases immunogenicity, enhances mRNA stability, and increases translational efficiency, resulting in more reliable and reproducible protein yield.
poly(A) tail: It contains an optimized poly(A) tail of approximately 100 nucleotides. This specific length is engineered to maximize transcript stability by resisting degradation and to synergize with the 5' cap for superior, sustained translation efficiency, ensuring robust protein yield in your applications.
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mRNA Length |
3550 nucleotides |
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Concentration |
~1 mg/mL |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
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General tips |
Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. |
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Shipping Condition |
ship with dry ice |
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