Ethacrynate Sodium

Ethacrynate Sodium (CAS No.: 6500-81-8) is the sodium salt form of ethacrynic acid and is commonly categorized as a small molecule pharmacological probe used in renal physiology, inflammation biology, and mechanism based drug discovery research, with functional relevance as an inhibitor of glutathione S transferases, a suppressor of NF kappa B associated signaling activity, a modulator of leukotriene formation, and an inhibitor of L type voltage dependent and store operated calcium entry pathways in smooth muscle related systems; in transporter focused studies it is widely described as interfering with sodium potassium chloride cotransport processes in nephron associated segments including the ascending limb and, to a lesser extent, proximal and distal tubular contexts, supporting its use as a research tool for ion transport and cellular homeostasis investigations. Reported in vitro effects are generally characterized at micromolar to low millimolar concentrations depending on assay format, enzyme abundance, and cell type, while pathway modulation in cellular systems is often observed from low micromolar to mid micromolar ranges, with exact exposure conditions varying across platforms and therefore requiring protocol specific optimization. In biomedical research workflows, this compound is frequently applied in cell based oxidative stress and inflammatory signaling models, transporter and calcium flux assays, and in vivo exploratory models of edema and tissue inflammatory responses to interrogate target engagement and downstream phenotypes, and the concentrations or doses used in experiments typically depend on specific experimental designs and research objectives.