Endogenous Peroxidase Blocking Buffer

Endogenous peroxidase is present in many cells and tissues, such as red blood cells, kidney, and liver tissues. Its presence can lead to high background or false-positive results when using peroxidase-based detection methods. Therefore, it is recommended to block the samples using an appropriate peroxidase blocking buffer prior to staining to eliminate interference from endogenous peroxidase.

This product is a ready-to-use formulation that has been extensively tested and optimized, providing excellent blocking performance that effectively reduces staining background without compromising specific signals. In many cases, its blocking efficacy surpasses that of conventional hydrogen peroxide solutions. It is primarily used for blocking endogenous peroxidase activity in tissues or cells during immunohistochemistry (IHC), immunocytochemistry (ICC), and in situ hybridization (ISH).

Based on a volume of 100 μL of blocking buffer per sample, one 100 mL package is sufficient for 1,000 samples.