Dihydroethidium
Dihydroethidium (CAS: 104821-25-2), also known as hydroethidine, is a cell-permeable fluorescent probe widely utilized for detecting superoxide anions (O??) in live cells. After entering cells, dihydroethidium is oxidized by intracellular superoxide anions to ethidium, which subsequently intercalates into DNA, emitting detectable red fluorescence (excitation/emission: 518/605 nm). Unoxidized dihydroethidium exhibits blue fluorescence (355/420 nm). The intensity of the red fluorescence correlates directly with intracellular O?? concentration. Thus, dihydroethidium serves as a valuable tool in evaluating oxidative stress and studying associated physiological/pathological processes, including apoptosis, cell proliferation, cardiovascular pathologies, diabetes, and cancer.
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| Storage | Store at -20°C for 12 months. |
| M.Wt | 315.41 |
| Cas No. | 104821-25-2 |
| Formula | C21H21N3 |
| Solubility | ≥31.5 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | 5-ethyl-6-phenyl-5,6-dihydrophenanthridine-3,8-diamine |
| SDF | Download SDF |
| Canonical SMILES | NC1=CC2=C(C3=C(C=C(N)C=C3)N(CC)C2C4=CC=CC=C4)C=C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
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