Dihydroethidium
Catalog No.
C3807
Dihydroethidium (DHE), is a peroxide indicator. It penetrates cell membranes to form fluorescent protein complexes that fluoresce blue.
Featured Products
Dihydroethidium (CAS: 104821-25-2), also known as hydroethidine, is a cell-permeable fluorescent probe widely utilized for detecting superoxide anions (O??) in live cells. After entering cells, dihydroethidium is oxidized by intracellular superoxide anions to ethidium, which subsequently intercalates into DNA, emitting detectable red fluorescence (excitation/emission: 518/605 nm). Unoxidized dihydroethidium exhibits blue fluorescence (355/420 nm). The intensity of the red fluorescence correlates directly with intracellular O?? concentration. Thus, dihydroethidium serves as a valuable tool in evaluating oxidative stress and studying associated physiological/pathological processes, including apoptosis, cell proliferation, cardiovascular pathologies, diabetes, and cancer.
- 1. Yonghu Chen, Wenjing Yu, et al. "Platanoside prevents ferroptosis in acute lung injury through Keap1 degradation-mediated activation of the Nrf2/GPX4 axis." Int Immunopharmacol. 2025 Nov 25;168(Pt 2):115933 PMID: 41297346
- 2. Zhaoran Wang, Meiyu Wang, et al. "Glabridin‐Gold (I) Complex as a Novel Immunomodulatory Agent Targeting TrxR and MAPK Pathways for Synergistic Enhancement of Antitumor Immunity." Adv Sci (Weinh). 2025 Aug 21:e04729 PMID: 40842087
- 3. Qian Liu, Ai Zhang, et al. "The Immunodeficiency Profile of Lymphocytes in the Patient with Moesin Gene Mutation During Different Infection." J Clin Immunol. 2025 Aug 11;45(1):122 PMID: 40788322
- 4. Zhijing He, Yingying Li, et al. "A cerium-based ferroptosis/PANoptosis nano-inducer for photothermal-enhanced combination therapy of breast cancer." Chemical Engineering Journal Volume 516, 15 July 2025, 163961
- 5. Jiazhen Zhang, Qinfen Li, et al. "Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1." Mechanobiol Med. 2025 Mar 26;3(2):100129 PMID: 40395775
- 6. Tingzi Yu, Cong Ding, et al. "SIRT7-mediated NRF2 deacetylation promotes antioxidant response and protects against chemodrug-induced liver injury." Cell Death Dis. 2025 Apr 1;16(1):232 PMID: 40169535
- 7. Yonghu Chen, Xilin Wu, Zhe Jiang, Xuezheng Li et al. "KAE ameliorates LPS-mediated acute lung injury by inhibiting PANoptosis through the intracellular DNA-cGAS-STING axis." Front Pharmacol. 2025 Jan 7;15:1461931 PMID: 39840115
- 8. Yao-lei Ma, Xiao-wei Huo, Colin Wong. "Salvianolic acid A targets glutamic-oxaloacetic transaminase 2 to ameliorate doxorubicin-induced myocardial oxidative injury by activating malate-aspartate NADH …." Phytomedicine Volume 148, 25 November 2025, 157492
- 9. Xue Han, Yilan Song, et al. "Mechanism of miR-130b-3p in relieving airway inflammation in asthma through HMGB1-TLR4-DRP1 axis." Research Square. 13 Sep, 2024
- 10. Qi Xue, Jie Li, et al. "Nrf2 activation by pyrroloquinoline quinone inhibits natural aging‐related intervertebral disk degeneration in mice." Aging Cell. 2024 May 23:e14202 PMID: 38780001
- 11. Xiaohan Liu, Hong Cui, et al. "miR-128-3p alleviates airway inflammation in asthma by targeting SIX1 to regulate mitochondrial fission and fusion." Int Immunopharmacol. 2024 Mar 30:130:111703 PMID: 38422767
- 12. Muzhe Li, Yuanyuan Niu, et al. "Astragaloside IV alleviates macrophage senescence and d-galactose-induced bone loss in mice through STING/NF-κB pathway." Int Immunopharmacol. 2024 Mar 10:129:111588 PMID: 38290207
- 13. Yaping Wang, Min Wang, et al. "SERCA2 dysfunction triggers hypertension by interrupting mitochondrial homeostasis and provoking oxidative stress." Free Radic Biol Med. 2024 Feb 20:212:284-294 PMID: 38163553
- 14. Chen K, Qiu P, et al. "Pseurotin A Inhibits Osteoclastogenesis and Prevents Ovariectomized-Induced Bone Loss by Suppressing Reactive Oxygen Species." Theranostics. 2019 Feb 28;9(6):1634-1650 PMID: 31037128
| Storage | Store at -20°C for 12 months. |
| M.Wt | 315.41 |
| Cas No. | 104821-25-2 |
| Formula | C21H21N3 |
| Solubility | ≥31.5 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | 5-ethyl-6-phenyl-5,6-dihydrophenanthridine-3,8-diamine |
| SDF | Download SDF |
| Canonical SMILES | CCN(C1c2ccccc2)c(cc(cc2)N)c2-c(cc2)c1cc2N |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
Chemical structure
Related Biological Data
