Cy3 Bis NHS ester
Cy3 Bis NHS ester is a reactive fluorescent dye for general labeling of protein amine groups. Cy3 Bis NHS ester includes two reactive groups on one dye molecule. The N-hydroxysuccinimde (NHS) ester is the most popular amine reactive group, used for capturing the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Appling NHS chemistry allows one to conjugate a variety of fluorescent probes (e.g. Cy3, a traditional orange-fluorescent label which is highly water soluble) to primary amines. Thus, target biological compounds can be visualized by Cy3 fluorescence (~550 nm excitation, ~570 nm emission) monitored by various fluorometers, imagers, and microscopes.
References:
1. Nanda JS, Lorsch JR. Labeling a protein with fluorophores using NHS ester derivitization. Methods in Enzymology, 2014, 536: 87-94.
2. Dyla M, Andersen JL, Kjaergaard M, et al. Engineering a Prototypic P-type ATPase Listeria monocytogenes Ca2+-ATPase 1 for Single-Molecule FRET Studies. Bioconjugate Chemistry, 2016, 27(9): 2176-2187.
| Storage | Store at -20°C |
| M.Wt | 932.99 |
| Cas No. | 146397-20-8 (free acid) |
| Formula | C43H49N4NaO14S2 |
| Synonyms | Sulfo-Cyanine 3 Bis-NHS ester, Sodium Salt; Sulfo Cy3 Bis NHS ester, Sodium Salt |
| Solubility | insoluble in EtOH; ≥41 mg/mL in DMSO; ≥54.6 mg/mL in H2O |
| Chemical Name | sodium 1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-((1E,3E)-3-(1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate |
| Canonical SMILES | CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CCCCCC(ON3C(CCC3=O)=O)=O)(C)C2=C/C=C/C4=[N+](CCCCCC(ON5C(CCC5=O)=O)=O)C(C=CC(S(=O)([O-])=[O-])=C6)=C6C4(C)C.[Na+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 555 | Emission max (nm) | 569 |
| Extinction Coefficient (M-1cm-1) | 150000 | Quantum Yield | 0.15 |
| CF260 | 0.07 | CF280 | 0.073 |
| Filter Set | Cy3/TRITC | Laser | 532 nm |
Quality Control & MSDS
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Chemical structure
