Coumarin Boronic Acid pinacolate ester

Coumarin Boronic Acid pinacolate ester (CAS No.: 190788-61-5) is a coumarin-based fluorescent probe widely employed in biomedical research for the detection of reactive oxygen and nitrogen species and the monitoring of intracellular biochemical processes. As a boronate-functionalized derivative with improved solubility, it undergoes nucleophilic oxidation reactions with species such as hydrogen peroxide, hypochlorous acid, peroxynitrite, and related hydroperoxides, leading to conversion into a highly fluorescent coumarin product, thereby enabling sensitive and selective detection of oxidative stress in biological systems. In addition to its reactivity toward oxidants, this probe has been utilized to investigate cellular glycosylation states through interactions with carbohydrate-related biomolecules, supporting studies of cell signaling, metabolism, and disease-associated glycosylation changes. Its mechanism of action is based on oxidation-triggered fluorescence activation, making it a valuable tool for probing redox-regulated pathways and inflammatory processes. In vitro applications typically employ concentrations in the low micromolar range, although optimal conditions vary depending on assay design and cell type. Coumarin Boronic Acid pinacolate ester is commonly used in live-cell imaging, high-content screening, and biochemical assays to elucidate oxidative stress dynamics, assess cellular responses to stimuli, and facilitate drug discovery efforts targeting redox imbalance and related pathological mechanisms.