Celestine Blue Stain Solution

H&E staining, a combination of Hematoxylin and Eosin, is the most widely used staining technique in pathology and histology. During Van Gieson staining, due to the effect of acid, most of the hematoxylin color is washed out, making the nuclei barely distinguishable. To address this issue, the use of Iron Hematoxylin or Celestine Blue can effectively resist the effect of acid, achieving ideal staining results.

Celestine Blue Staining Solution consists mainly of 0.5% Celestine Blue B and is frequently used in conjunction with Alum Hematoxylin. The advantage of this combination is that Celestine Blue B possesses excellent acid resistance, and the ferric salts can enhance the binding force of Alum Hematoxylin to the nucleus, resulting in deeper nuclear staining. Therefore, this combination not only achieves the purpose of acid resistance but also ensures that the hematoxylin color is not easily washed out.