Cefradine

Cefradine (CAS No.: 38821-53-3) is a semisynthetic first generation cephalosporin within the beta lactam antibacterial class that is widely used as a reference small molecule in microbiology and anti infective research, with primary relevance to studies of bacterial cell wall biogenesis, beta lactam susceptibility, and resistance evolution across Gram positive and selected Gram negative organisms; functionally, its canonical mechanism is inhibition of bacterial penicillin binding proteins involved in peptidoglycan cross linking, leading to loss of cell wall integrity and bactericidal effects in susceptible strains, while published research descriptions also indicate activity in inflammation focused models through direct inhibition of TOPK signaling in ultraviolet induced skin inflammation paradigms, supporting a secondary use case in pathway interrogation; consistent with first generation cephalosporin behavior, in vitro potency is generally characterized in microbiological assays by minimum inhibitory concentrations that commonly fall in the low microgram per milliliter to higher microgram per milliliter range depending on species, strain background, inoculum, and beta lactamase status, and target engagement in biochemical or cell based pathway studies is typically observed across nanomolar to micromolar to low millimolar experimental windows according to assay design rather than a single universal value; in biomedical discovery workflows, Cefradine is therefore commonly applied in susceptibility profiling, resistance mechanism mapping, combination screening with beta lactamase related modulators or orthogonal antibacterials, and exploratory cell or animal model studies of infection biology and host response, where the concentrations or doses used in experiments typically depend on specific experimental designs and research objectives, and all use should remain restricted to controlled laboratory research settings only.