Caspase-2 Fluorometric Assay Kit
Cysteine-containing aspartate proteolytic enzymes (Caspase) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Caspase-2 is considered to be an upstream initiator of apoptosis signaling, and activation of caspase-2 can trigger a series of downstream events, including activation of executor caspases (e.g., caspase-3, caspase-7), thereby promoting the execution of apoptosis.
Caspase-2 Fluorometric Assay Kit provides a convenient and simple way for detecting the VDVAD-dependent caspase activity. VDVAD-AFC (AFC:7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of VDVAD-AFC by Caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified by using a microplate reader or a fluorometer. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control determines the fold increase in Caspase-2 activity.
| Components |
K2016-25T |
K2016-100T |
K2016-200T |
K2016-400T |
|
Cell Lysis Buffer |
25 mL |
100 mL |
100 mL |
100 mL |
|
2X Reaction Buffer |
2 mL |
4 X 2 mL |
16 mL |
32 mL |
|
VDVAD-AFC (1 mM) |
125 µL |
500 µL |
2 X 0.5 mL |
2 X 1 mL |
|
DTT (1 M) |
100 µL |
400 µL |
400 µL |
400 µL |
|
Store the kit at -20°C, stable for 6 months. VDVAD-AFC (1 mM) should be stored away from light. |
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| Features |
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