BRITE-338733

BRITE-338733 (CAS No.: 503105-88-2) is a small-molecule inhibitor of bacterial RecA ATPase, a key enzyme involved in homologous recombination and DNA repair pathways, and is widely used in studies of antimicrobial sensitization and DNA damage responses. It effectively suppresses RecA-mediated ATP hydrolysis with activity in the low micromolar range, thereby impairing bacterial DNA repair mechanisms and potentially enhancing susceptibility to antibacterial agents. In addition to its activity against bacterial RecA, BRITE-338733 has been shown to inhibit the ATP hydrolysis function of the RSC chromatin remodeling complex through interactions with its ATP-binding pocket and DNA, exhibiting submicromolar potency in biochemical assays. The compound also demonstrates cytotoxic effects in human cancer cell lines, including MCF-7 and MDA-MB-231, supporting its utility in oncology-related research. Collectively, these properties position BRITE-338733 as a valuable chemical probe for investigating RecA-dependent processes, chromatin remodeling mechanisms, and the interplay between DNA repair and cell viability. Experimental applications typically involve in vitro enzymatic assays and cell-based models, with concentrations adjusted according to specific study designs and biological contexts.