Annexin V-PE Reagent

Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V.

Annexin V-PE Reagent uses a fluorescent conjugate of Annexin V that can easily detect PS on the cell surface after initiating apoptosis. The one-step staining procedure needs only 15-30 minutes. The result can be analyzed by fluorescence microscopy or by flow cytometry. Use of this reagent also needs to purchase 10X Binding Buffer (Cat. No. K2284). Or directly purchase Annexin V-PE Apoptosis Kit (Cat. No. K2281), which provides the binding buffer.