Acridine Orange 10-Nonyl Bromide
Acridine Orange 10-Nonyl Bromide (NAO), a lipophilic cationic derivative of acridine orange, readily traverses live-cell membranes and binds specifically to cardiolipin, an anionic phospholipid enriched in the inner mitochondrial membrane. NAO exhibits green fluorescence (Ex/Em ~496/524 nm). Notably, localized probe accumulation promotes oligomeric stacking with cardiolipin, inducing a spectral red shift with peak excitation/emission at ~450/640 nm.
Unlike potential-sensitive probes such as Rhodamine 123 and JC-1, NAO accumulation is relatively independent of mitochondrial membrane potential (ΔΨₘ), enabling robust quantification of mitochondrial mass and assessment of inner membrane integrity. Consequently, NAO is widely employed for live-cell mitochondrial morphotyping, monitoring cardiolipin dynamics during apoptosis, flow cytometric analysis of mitochondrial content, and staining Gram-negative bacteria.
| Physical Appearance | Powder |
| Storage | Store at 4°C away from moisture and light for 2 years |
| M.Wt | 472.50 |
| Cas No. | 75168-11-5 |
| Formula | C26H38BrN3 |
| Solubility | Soluble in DMSO or DMF |
| Chemical Name | 3,6-bis(dimethylamino)-10-nonylacridin-10-ium bromide |
| Canonical SMILES | CN(C)C1=CC([N+](CCCCCCCCC)=C(C=C(N(C)C)C=C2)C2=C3)=C3C=C1.[Br-] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |