25-Hydroxyvitamin D2-d3

25-Hydroxyvitamin D2-d3 (CAS No.: 1217467-39-4) is the stable isotope-labeled counterpart of 25-hydroxyvitamin D2, mainly used for metabolomics analysis and drug development in biomedical research. As a metabolite of vitamin D2, 25-hydroxyvitamin D2 is produced in vivo through hepatic metabolism and is involved in the regulation of calcium and phosphorus metabolism and bone health. Since its concentration in blood can reflect the body’s vitamin D status, it is widely used to assess vitamin D levels. The deuterium labeling of 25-Hydroxyvitamin D2-d3 enables it to serve as an internal standard in liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, providing highly sensitive and highly specific quantitative detection, and is commonly used for accurate determination of 25-hydroxyvitamin D2 in biological samples such as serum and plasma. The dose or concentration used in experiments typically depends on the experimental design and research objectives.