(±)-Blebbistatin
Catalog No.
B6847
non-muscle myosin II ATPases inhibitor, cell-permeable
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(±)-Blebbistatin (CAS: 674289-55-5) is a cell-permeable small molecule inhibitor selectively targeting non-muscle myosin II (NM II) ATPase. It preferentially binds the myosin-ADP-Pi intermediate complex, delaying phosphate release and consequently preventing actin-activated ATPase activity and myosin II-mediated contraction. Blebbistatin inhibits several isoforms of myosin II across multiple species with IC50 values from approximately 0.5 to 5.0 μM, but exhibits significantly weaker inhibition towards smooth muscle myosin II and myosin isoforms I, V, and X. This inhibitor is widely applied in cellular research to reversibly modulate cytoskeletal dynamics, cell migration, adhesion, and morphology.
- 1. Huijing Wang, Catalina Ardila, et al. "Protrusion force and cell-cell adhesion play a critical role in collective tumor cluster migration." bioRxiv. 2024 Sep 22:2024.09.18.613706. PMID: 39345581
- 2. Wu, Xiaobo. "The role of the perinexus in Long QT Syndrome Type 3." Virginia Tech 2023-02-13
- 3. Ivan Y. Melnikov, Sergey A. Tyganov, et al. "Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle." Research Square. May 2nd, 2022.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 292.34 |
| Cas No. | 674289-55-5 |
| Formula | C18H16N2O2 |
| Solubility | ≥47.9 mg/mL in DMSO; ≥3.1 mg/mL in EtOH with ultrasonic; insoluble in H2O |
| Chemical Name | 3a-hydroxy-6-methyl-1-phenyl-3,3a-dihydro-1H-pyrrolo[2,3-b]quinolin-4(2H)-one |
| SDF | Download SDF |
| Canonical SMILES | OC12CCN(C1=NC3=C(C2=O)C=C(C=C3)C)C4=CC=CC=C4 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
Related Biological Data
