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Cy3 TSA Fluorescence System Kit

Catalog No.
K1051
used for signal amplification of in situ hybridization(ISH), immunohistochemistry(IHC), immunocytochemistry(ICC) and other related experiments
Grouped product items
SizePriceStock Qty
100-300 slides
$230.00
In stock
200-600 slides
$425.00
In stock

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Cy3 TSA Fluorescence System Kit
 

Protocol

Components and Storage

Complete Kit 100-300 slides 200-600 slides
1X Amplification Diluent 30 mL 60 mL
Cyanine 3 Tyramide (dry, dissolve in 60 uL DMSO) 1 tube 2 tubes
Blocking Reagent 6 g 12 g

Store Fluorescein Tyramide in the dark at -20°C and keep 1X Amplification Diluent and Blocking Reagen in the dark at 4°C.

Description

Tyramine Signal Amplification (TSA) from APExBIO technology increases sensitivity by a factor of 100 and enables detection of low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC) and in situ hybridization (FISH) applications.

The TSA Fluorescence Kit uses horseradish peroxidase (HRP) to directly catalyze the covalent deposition of the fluorophore adjacent to the immobilized enzyme. The labeling process is so fast (less than ten minutes) and the deposition label can be viewed directly under standard or confocal microscopy. TSA fluorescein can also be used in combination with anti-fluorescein.

TSA technology is used for bright field microscopy of enzyme conjugates and suitable chromogenic substrates. The use of TSA reagents results in a significant increase in sensitivity compared to standard assays while maintaining stable specificity and resolution. In addition, TSA reagents can significantly reduce the consumption of primary antibodies or probes. The fluorescent labeling signal of this kit (K1051) is Cyanine 3, which can be detected the signal by microscopy at the excitation and emission wavelengths (550nm/570nm).

Schematic diagram of tyramine signal amplification system

Label the cell or tissue sample with the primary and secondary antibodies using conventional methods. The horseradish peroxidase (HRP) conjugated to the second antibody catalyzes the conversion of the labeled tyramide to a reactive radical, and the tyramide radical is covalently bound to a nearby tyrosine residue to provide a high-density label.