|EZLink NHS-SS-Biotin Kit Amine-reactive biotinyltation kit|
Sample solution is provided at 25 µL, 10mM.
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Components and storage
|Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody|
|Streptavidin||10 mg||EZ-Link NHS-SS-Biotin||25 mg|
|HABA Solution||1 mL||PBS Pack (makes 500 mL)||1 pack|
|Sephadex G-25 in PD-10 Desalting Columns||8.3 mL, 10 columns|
|Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C.|
|Label or Dye:||Biotin||Product Size:||10 reactions|
|Labeling Scale:||1-10 mg||Labeling Target:||Proteins (General), Antibodies (General)|
Clearance of components and concentrations.
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• Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.
• Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.
• Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.
• Labeling Cell surface molecule—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.
• Reversible-Disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule.
• Medium length—Spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain
• Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.
NHS-SS-biotin (Succinimidyl-2-(biotinamido)-ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent that has a spacer arm of 24.3 A in length due to the existence of a cross-bridge within its chemical structure. The long spacer arm creates an increased length between a covalently modified molecule and the bicyclic biotin rings that greatly enhances the ability of the molecule to bind to avidin or streptavidin probes. The NHS ester group of NHS-SS-biotin reacts with the amine groups of proteins and other molecule forming an amide bond and releasing of sulfo-NHS. NHS-SS-Biotin is a water-insoluble molecule that requires the dissolution of organic solvents prior to be added to aqueous reactions.