Zalcitabine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 211.22 |
| Cas No. | 7481-89-2 |
| Formula | C9H13N3O3 |
| Solubility | insoluble in EtOH; ≥10.65 mg/mL in DMSO with gentle warming; ≥15.87 mg/mL in H2O with ultrasonic |
| Chemical Name | 4-amino-1-[(2R,5S)-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one |
| SDF | Download SDF |
| Canonical SMILES | C1CC(OC1CO)N2C=CC(=NC2=O)N |
| Shipping Condition | Ship with blue ice, or upon other requests. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. |
| Cell experiment:[1] | |
|
Cell lines |
3201 (feline lymphoid) cells, 81C (sarcoma-positive, leukemia-negative feline lung fibroblast) cells, and primary feline bone marrow cells |
|
Reaction Conditions |
0 ~ 384 μM zalcitabine |
|
Applications |
Zalcitabine (5 ~ 10 μM) inhibited feline leukemia virus (FeLV) infection of feline lymphoid cells by greater than 80%, while 6.07 ~ 12.13 μM zalcitabine was required to similarly inhibit infection of feline fibroblasts. However, 43 ~ 384 μM zalcitabine was needed to inhibit FeLV infection of primary bone marrow cells by greater than 80%. |
| Animal experiment:[1] | |
|
Animal models |
Cats challenged intravenously with FeLV 1 ~ 3 days after drug treatment began |
|
Dosage form |
22, 15, 10, and 5 mg/kg per h Administered by continuous intravenous infusion for 28 days |
|
Applications |
Doses of 22 and 15 mg/kg per h were extremely toxic, causing death in 8 of 10 cats. The 10 mg/kg per h dose was slightly toxic, causing chronic progressive thrombocytopenia over the 28-day treatment period. Of 10 cats given 10 or 5 mg of zalcitabine per kg per h, only one was completely protected from FeLV antigenemia. However, conversion to positive FeLV antigenemia status was delayed by 2 ~ 7 weeks in seven of nine remaining animals. |
|
Note |
The technical data provided above is for reference only. |
|
References: 1. Polas PJ, Swenson CL, Sams R, et al. In vitro and in vivo evidence that the antiviral activity of 2',3'-dideoxycytidine is target cell dependent in a feline retrovirus animal model. Antimicrobial Agents and Chemotherapy, 1990, 34(7): 1414-1421. |
|
Quality Control & MSDS
- View current batch:
Chemical structure
