Toggle Nav

YM201636

In stock
Catalog No.
B2189
PIKfyve inhibitor,potent and selective
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$100.00
In stock
5mg
$96.00
In stock
10mg
$170.00
In stock
50mg
$645.00
In stock
100mg
$1,100.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

YM201636 is a potent, selective and cell-permeable inhibitor of mammalian class III phosphatidylinositol phosphate kinase PIKfyve with IC50 value of 33nM [1].

The pyridofuropyrimidine compound, YM201636, is a small-molecule inhibitor of PIKfyve. In the in vitro assay, it inhibits PIKfyve with IC50 value of 33nM and shows no inhibition of yeast orthologue of PIKfyve. It inhibits PtdIns3P p110α and type Iα PtdInsP kinase with IC50 values of 3μM and > 2μM, respectively. The type IIγ PtdInsP kinase is found to be insensitive to YM201636. In serum-starved NIH3T3 cells, YM201636 inhibits PtdIns (3, 5) P2 production by 80% at concentration of 800nM. Acute treatment of YM201636 to MEFs, MDCK, MCF10A, COS7 and NIH3T3 cells causes the formation of large vesicular structures. Besides that, YM201636 is also found to reduce the Akt phosphorylation and GLUT4 cell surface translocation through inhibiting the insulin-dependent class I PI 3k activation [1, 2].

References:
[1] Jefferies H B J, Cooke F T, Jat P, et al. A selective PIKfyve inhibitor blocks PtdIns (3, 5) P2 production and disrupts endomembrane transport and retroviral budding. EMBO reports, 2008, 9(2): 164-170.
[2] Ikonomov O C, Sbrissa D, Shisheva A. YM201636, an inhibitor of retroviral budding and PIKfyve-catalyzed PtdIns (3, 5) P2synthesis, halts glucose entry by insulin in adipocytes. Biochemical and biophysical research communications, 2009, 382(3): 566-570.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt467.48
Cas No.371942-69-7
FormulaC25H21N7O3
Solubility≥11.68mg/mL in DMSO
Chemical Name6-amino-N-[3-(4-morpholin-4-ylpyrido[2,3]furo[2,4-b]pyrimidin-2-yl)phenyl]pyridine-3-carboxamide
SDFDownload SDF
Canonical SMILESC1COCCN1C2=NC(=NC3=C2OC4=C3C=CC=N4)C5=CC(=CC=C5)NC(=O)C6=CN=C(C=C6)N
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

Cell experiment [1, 2]:

Cell lines

Mouse 3T3L1 adipocytes, NIH3T3 cells

Preparation method

The solubility of this compound in DMSO is >11.7mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0-4 μM for 30 min or 800 nM for 2h

Applications

In mouse 3T3L1 adipocytes, YM201636 significantly inhibited both basal and insulin-activated 2DG uptake in a dose-dependent way. YM201636 (160 nM) nearly completely inhibited the net insulin effect with a 50% inhibition at 54 ± 4 nM. YM201636 at 800 nM produced a 45% inhibition of cell surface HA-GLUT4-eGFP accumulation and a 55% inhibition of Akt-Ser473 phosphorylation. In NIH3T3 cells, YM201636 at 800 nM decreased PtdIns(3,5)P2 production by 80%. YM201636 induced the vesiculation phenotype by affecting PIKfyve and PtdIns(3,5)P2 production.

References:

[1] Jefferies H B J, Cooke F T, Jat P, et al. A selective PIKfyve inhibitor blocks PtdIns (3, 5) P2 production and disrupts endomembrane transport and retroviral budding. EMBO reports, 2008, 9(2): 164-170.

[2]. Ikonomov O C, Sbrissa D, Shisheva A. YM201636, an inhibitor of retroviral budding and PIKfyve-catalyzed PtdIns (3, 5) P2synthesis, halts glucose entry by insulin in adipocytes. Biochemical and biophysical research communications, 2009, 382(3): 566-570.

Biological Activity

Description YM201636 is a selective inhibitor of PIKfyve with an IC50 value of 33 nM.
Targets PIKfyve p110α        
IC50 33 nM 3.3 μM        

Quality Control

Chemical structure

YM201636