Tetrazole

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: Not available.
Tetrazole is the simplest compound belonging to tetrazoles family, a class of synthetic organic heterocyclic compound consists of a 5-member ring of four nitrogen and one carbon atom. Tetrazoles have commonly been believed to have similar properties to carboxylic acids, and therefore make themselves excellent bioisosteres. Moreover, several pharmaceutical agents, for instance, losartan and candesartan (Angiotensin II receptor blockers), are considered as tetrazoles. Tetrazoles are often adopted in medicinal chemistry as a substitute for carboxylic acids because they share very similar proton dissociation properties. [1]
In vitro: The acidity constant of tetrazole was detected and reported as 4.9. Four of the six angiotension II receptor antagonists clinically effective for treating hypertension contained a tetrazole. MTT, dimethyl thiazolyl diphenyl tetrazolium bromide, was regarded as a well-known tetrazole applied to quantify the respiratory activity of live cells. Some tetrazoles could also be used in DNA assays. [2]
In vivo: So far, no in vivo data has been reported.
Clinical trial: So far, no clinical trial has been conducted.
References:
[1]Berner S, Mühlegger K and Seliger H. Studies on the role of tetrazole in the activation of phosphoramidites. Nucleic Acids Res. 1989 Feb; 17 (3): 853–864
[2]Satchell JF and Smith BJ. Calculation of aqueous dissociation constants of 1,2,4-triazole and tetrazole: A comparison of solvation modelsy. Phys Chem Chem Phys. 2002; 4: 4314-8.
Storage | Store at -20°C |
M.Wt | 70.1 |
Cas No. | 288-94-8 |
Formula | CH2N4 |
Solubility | ≥13.1 mg/mL in DMSO; ≥22.8 mg/mL in EtOH; ≥28 mg/mL in H2O |
Chemical Name | 2H-tetrazole |
SDF | Download SDF |
Canonical SMILES | C1=NNN=N1 |
Shipping Condition | Ship with blue ice, or upon other requests. |
General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
