Determination of lipase activity
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For determination of lipase activity, lysates are incubated with a substrate containing radiolabeled [9,10-3H(N)]-triolein. Subsequently, FA are extracted and quantitated by liquid scintilation counting. Data are presented as mean±S.D. of triplicate determinations and representative for at least three independent experiments.
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References:
1.Mayer, N., Schweiger, M., Romauch, M., Grabner, G. F., Eichmann, T. O., Fuchs, E., Ivkovic, J., Heier, C., Mrak, I., Lass, A., Hofler, G., Fledelius, C., Zechner, R., Zimmermann, R. and Breinbauer, R. (2013) Development of small-molecule inhibitors targeting adipose triglyceride lipase. Nat Chem Biol. 9, 785-787
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