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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
(5Z)-7-Oxozeaenol is a natural resorcylic lactone derived from fungi that selectively inhibits purified TAK-1 (IC50 = 8.1 nM) without significantly affecting the kinase activities of other MAPKKK family members.
In cells, (5Z)-7-Oxozeaenol is reported to irreversibly block IL-1-induced activation of TAK-1, NF-κB, and JNK/p38 with IC50 values of 65-83 nM.
(5Z)-7-Oxozeaenol has also been shown to inhibit IL-1-induced COX-2 production in cells and to relieve ear swelling induced by picryl chloride in a mouse model of COX-2-mediated inflammation.
Reference:
[1]. Ninomiya-Tsuji J, Kajino T, Ono K, et al. A resorcylic acid lactone, 5Z-7-oxozeaenol, prevents inflammation by inhibiting the catalytic activity of TAK1 MAPK kinase kinase. J. Biol. Chem, 2003, 278(20): 18485-18490.
Cell lines
Mouse embryonic fibroblast cells
Reaction Conditions
500 nM (5Z)-7-oxozeaenol for 17.5 h incubation
Applications
In cell culture, (5Z)-7-oxozeaenol blocked interleukin-1-induced activation of TAK1, JNK/p38 MAPK, IκB kinases, and NF-κB, resulting in inhibition of cyclooxgenase-2 production.
Animal models
Mice repeatedly sensitized by picryl chloride (PC) and subsequently challenged with PC
Dosage form
10 μl of (5Z)-7-oxozeaenol (1 mg/ml)
Painted on each ear 2 h before and 4 h after the second PC challenge (total of twice) or every hour three times before and 4 h after the second PC challenge (total of four times)
When (5Z)-7-oxozeaenol was administrated to the PC-challenged ear, ear swelling was reduced by up to 50% of that of the control ear treated with vehicle. Thus, (5Z)-7-oxozeaenol was able to prevent inflammation by inhibiting the catalytic activity of TAK1 MAPKKK.
Note
The technical data provided above is for reference only.
References:
1. Ninomiya-Tsuji J, Kajino T, Ono K, et al. A resorcylic acid lactone, 5Z-7-oxozeaenol, prevents inflammation by inhibiting the catalytic activity of TAK1 MAPK kinase kinase. Journal of Biological Chemistry, 2003, 278(20): 18485-18490.