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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
human embryonic kidney (HEK293) cells (with high expression levels of the cloned murine 2A-adrenoceptor ,α2C-adrenoceptor and α2B -adrenoceptor subtypes)
Preparation method
The solubility of this compound in DMSO is >12.2mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
10μM for 20 min
Applications
In membranes from HEK293 cells transfected with α2-receptors, etomidate inhibited binding of the α2-antagonist, [3H]RX821002, with higher potency from α2B- and α2C-receptors than from α2A-receptors.Etomidate activated mitogen-activated protein kinase phosphorylation via α2B -receptors. In α2B-receptor–expressing HEK293 cells, stimulated with 10μM etomidate for 20 min rapidly increased phosphorylation of the extracellular signal-related kinases ERK1/2.
Animal models
α2Adrenoceptor-deficient Mice, α2Bdrenoceptor-deficient Mice
Dosage form
intraperitoneal injection ,5–50 mg/kg body weight
Application
These results showed that 27% of the mice lost the reflex at 10 mg/kg etomidate and all mice transiently lost the righting reflex at 20mg/kg and higher etomidate doses. After injection of etomidate at 30 mg/kg, the righting reflex disappeared in wild-type and α2 A-deficient mice at similar times after intraperitoneal injection. On intravenous injection of etomidate at 30 mg/kg, wild-type mice showed a rapid and transient hypertensive response that was completely absent in mice lacking α2B-receptors.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Paris A, Philipp M, Tonner PH., et al. Activation of alpha 2B-adrenoceptors mediates the cardiovascular effects of etomidate. Anesthesiology. 2003 Oct;99(4):889-95.