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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
The parental human colon cancer cell line KM12C and its two highly metastatic derivatives KM12SM and KM12L4a
Reaction Conditions
2.5 μg/ml 7-ethyl-10-hydroxycamptothecin for 4, 24 or 48 h incubation
Applications
The exposure of the cells to 7-ethyl-10-hydroxycamptothecin induced S-phase and G2 arrest and the KM12L4a cells had the highest response in a time-dependent manner. The rates of apoptosis in the KM12SM and KM12L4a cell lines were increased time-dependently, though there was no such change in the KM12C cells. In conclusion, the effect of 7-ethyl-10-hydroxycamptothecin on the colon cancer cell lines was mediated via conducting S-phase and G2 arrest and apoptosis. This effect was found in the cell lines with higher metastatic potentials, indicating that 7-ethyl-10-hydroxycamptothecin can be used to treat advanced colon cancers.
Note
The technical data provided above is for reference only.
References:
1. Wallin A, Svanvik J, Holmlund B, et al. Anticancer effect of SN-38 on colon cancer cell lines with different metastatic potential. Oncology Reports, 2008, 19(6): 1493-1498.