JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
U2OS cells, Vero cells
Preparation method
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
2 μM
Applications
Nanchangmycin was modestly toxic but very efficacious in inhibiting viral infection. Nanchangmycin blocked ZIKV infection with low toxicity in the sub-micromolar range. Nanchangmycin potently blocked ZIKV infection across cell types. The IC50 values for infection were between 0.1 and 0.4 μM. Nanchangmycin inhibited DENV across cell types. In Vero cells, treatment with nanchangmycin reduced viral titers by 4-log. Nanchangmycin blocked an early step in the viral life cycle. In U2OS cells, treatment with nanchangmycin (2 μM) blocked infection by an additional flavivirus (WNV, Kunjin) or alphaviruses (CHIKV, 181/25; SINV, HRsp). In HBMECs, nanchangmycin effectively blocked ZIKV infection in HUVECs and UtMECs. Nanchangmycin potently inhibited ZIKV infection in primary placental fibroblast cells.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Rausch K, Hackett B A, Weinbren N L, et al. Screening bioactives reveals nanchangmycin as a broad spectrum antiviral active against Zika virus[J]. Cell reports, 2017, 18(3): 804-815.