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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Golgicide A is a potent, reversible and highly specific GBF1 inhibitor.GBF1, the ArfGEF responsible for Arf1 activation and COPI recruitment to cis-Golgi membranes, plays an important role in coordinating bidirectional transport and maintaining structural integrity of the Golgi [1].Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM in Vero cells. Immunofluoresence experiments demonstrates that Golgicide A causes complete dispersal of the medial-Golgi markers giantin and the cis-Golgi marker GM130 and results in a rapid redistribution of COPI from the Golgi. Also, Golgicide A causes a decrease in GBF1-mediated Arf1 activation, impairs retrograde toxin transport and arrests secretion of soluble and membrane-anchored proteins [1]. Golgicide A decreases HCV RNA levels and causes redistribution of NS5A in FLRP1 cells and J6/JFH1 cells. In addition, Golgicide A causes accumulation of infectious viral particles in J6/JFH1 cells [2]. References:[1] Sáenz JB, Sun WJ, Chang JW, et al. Golgicide A reveals essential roles for GBF1 in Golgi assembly and function. Nat Chem Biol, 2009, 5(3): 157-165.[2] Matto M, Sklan EH, David N, et al. Role for ADP ribosylation factor 1 in the regulation of hepatitis C virus replication. J Virol, 2011, 85(2): 946-956.
Cell lines
The human hepatoma cell line Huh7 (Huh7.5 cells were electroporated with the J6/JFH1 RNA and were transfected with an NS5A-encoding plasmid.)
Preparation method
The solubility of this compound in DMSO is >13mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
10μM for 4, 24, and 48 h.
Applications
Treatment of cells with golgicide A, a drug that specifically inhibits GBF1, had been shown to decrease HCV (hepatitis C virus) RNA replication. Thus, golgicide A treatments diminished Arf1 (ADP-ribosylation factor 1) and HCV RNA levels in the JFH1-HCVcc cell system. In J6/JFH1 cells, some of the viral NS proteins migrated from speckle-like organelles to the peripheries of LDs in response to Arf1 inhibition by golgicide A treatment. Golgicide A treatments also decreased HCV RNA levels and cause redistribution of NS5A (a nonstructural protein) and accumulation of infectious viral particles.
References:
[1]. Matto M, Sklan EH, David N., et al.Role for ADP ribosylation factor 1 in the regulation of hepatitis C virus replication. J Virol, 2011, 85(2): 946-956.