Granzyme B Inhibitor Z-AAD-CH2Cl
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Z-AAD-CH2Cl (Z-AAD-CMK) is a cell-permeable and irreversible inhibitor of granzyme B .
Granzyme B is an apoptosis-inducing factor that expressed in natural killer (NK) cells, the granules of cytotoxic lymphocytes (CTLs) and cytotoxic T cells, and plays an important role in inducing inflammation and extracellular matrix remodelling .
Z-AAD-CH2Cl (Z-AAD-CMK) is a cell-permeable and irreversible granzyme B inhibitor. In human C-28/12 chondrocytes expressed the surface antigens of NK cells, Z-AAD-CMK dose-dependently inhibited chondrocyte cytotoxicity against K562 cells that was dependent on granzyme B . In the oral squamous cell carcinoma cell line OSC-3, Z-AAD-CMK reduced interleukin-2-activated lymphocytes (LAK cell)-enhanced caspase-3 activity to approximately 70% and inhibited DNA fragmentation by approximately 20% of the control. Z-AAD-CMK also inhibited the increase of reactive oxygen intermediates (ROI) induced by LAK cells to approximately half of the control . In Tc1 cells, Z-AAD-CMK inhibited apoptosis .
. Saito S, Murakoshi K, Kotake S, et al. Granzyme B induces apoptosis of chondrocytes with natural killer cell-like cytotoxicity in rheumatoid arthritis. J Rheumatol, 2008, 35(10): 1932-1943.
. Yamamoto T, Yoneda K, Ueta E, et al. Enhanced apoptosis of squamous cell carcinoma cells by interleukin-2-activated cytotoxic lymphocytes combined with radiation and anticancer drugs. Eur J Cancer, 2000, 36(15): 2007-2017.
. Gorak-Stolinska P, Truman JP, Kemeny DM, et al. Activation-induced cell death of human T-cell subsets is mediated by Fas and granzyme B but is independent of TNF-alpha. J Leukoc Biol, 2001, 70(5): 756-766.
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||(Z)-2-((E)-((benzyloxy)(hydroxy)methylene)amino)-N-((Z)-1-((5-chloro-1-methoxy-1,4-dioxopentan-3-yl)imino)-1-hydroxypropan-2-yl)propanimidic acid|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.|