Granzyme B Inhibitor Z-AAD-CH2Cl
Z-AAD-CH2Cl (Z-AAD-CMK) is a cell-permeable and irreversible inhibitor of granzyme B .
Granzyme B is an apoptosis-inducing factor that expressed in natural killer (NK) cells, the granules of cytotoxic lymphocytes (CTLs) and cytotoxic T cells, and plays an important role in inducing inflammation and extracellular matrix remodelling .
Z-AAD-CH2Cl (Z-AAD-CMK) is a cell-permeable and irreversible granzyme B inhibitor. In human C-28/12 chondrocytes expressed the surface antigens of NK cells, Z-AAD-CMK dose-dependently inhibited chondrocyte cytotoxicity against K562 cells that was dependent on granzyme B . In the oral squamous cell carcinoma cell line OSC-3, Z-AAD-CMK reduced interleukin-2-activated lymphocytes (LAK cell)-enhanced caspase-3 activity to approximately 70% and inhibited DNA fragmentation by approximately 20% of the control. Z-AAD-CMK also inhibited the increase of reactive oxygen intermediates (ROI) induced by LAK cells to approximately half of the control . In Tc1 cells, Z-AAD-CMK inhibited apoptosis .
. Saito S, Murakoshi K, Kotake S, et al. Granzyme B induces apoptosis of chondrocytes with natural killer cell-like cytotoxicity in rheumatoid arthritis. J Rheumatol, 2008, 35(10): 1932-1943.
. Yamamoto T, Yoneda K, Ueta E, et al. Enhanced apoptosis of squamous cell carcinoma cells by interleukin-2-activated cytotoxic lymphocytes combined with radiation and anticancer drugs. Eur J Cancer, 2000, 36(15): 2007-2017.
. Gorak-Stolinska P, Truman JP, Kemeny DM, et al. Activation-induced cell death of human T-cell subsets is mediated by Fas and granzyme B but is independent of TNF-alpha. J Leukoc Biol, 2001, 70(5): 756-766.
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||(Z)-2-((E)-((benzyloxy)(hydroxy)methylene)amino)-N-((Z)-1-((5-chloro-1-methoxy-1,4-dioxopentan-3-yl)imino)-1-hydroxypropan-2-yl)propanimidic acid|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|