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Cy7 maleimide (non-sulfonated)
Labeling of cysteine residues, as well as other thiolated molecules

Catalog No.A8141
Size Price Stock Qty
Cy7 maleimide 5mg
$210.00
In stock
Cy7 maleimide 25mg
$410.00
In stock

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Email: [email protected]

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Sample solution is provided at 25 µL, 10mM.

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Chemical structure


Cy7 maleimide (non-sulfonated)

Related Biological Data

Cy7 maleimide (non-sulfonated)

Cy7 maleimide (non-sulfonated) Dilution Calculator

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Chemical Properties

Cas No. SDF Download SDF
Chemical Name 6-[(2E)-3,3-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,3,3-trimethylindol-1-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]indol-1-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide
Canonical SMILES CC1(C2=CC=CC=C2[N+](=C1C=CC3=CC(=CC=C4C(C5=CC=CC=C5N4CCCCCC(=O)NCCN6C(=O)C=CC6=O)(C)C)CCC3)C)C
Formula C43H51ClN4O3 M.Wt 707.34
Solubility soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water Storage 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Spectral properties

Excitation max (nm) 750 Emission max (nm) 773
Extinction Coefficient (M-1cm-1) 199000 Quantum Yield 0.3
CF260 0.022 CF280 0.029

Application

Non-sulfonated cyanines are available for the labeling of many targets including:

• Soluble proteins, which are tolerant to addition of organic co-solvent • Fluorescence polarization (FP)
• Antibodies (with 5-10% of DMSO/DMF) • Fluorescence resonance energy transfer (FRET)
• DNA and oligonucleotides • Time-resolved fluorescence energy transfer (TR-FRET)
• Peptides • Fluorescence intensity (FI)
• Many small molecules  
• Reactions in organic media (dichloromethane, acetonitrile)  

Background

Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. Labeled proteins thus obtained are used in NIR bioimaging applications. In tissues or in live organism, NIR imaging systems can be used to visualize distribution of labeled proteins. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In splenocytes, for ease of detection, P22 particles were labeled internally with Cy7-maleimide. P22-Cy7 alone or P22-Cy7 decorated with DecSelf was incubated with splenocytes at 37℃ to encourage phagocytosis [1].

Reference:
[1].  Schwarz, B.; Madden, P.; Avera, J.; Gordon, B.; Larson, K.; Miettinen, H.; Uchida, M.; LaFrance, B.; Basu, G.; Rynda-Apple, A.; Douglas, T. Symmetry Controlled, Genetic Presentation of Bio-Active Proteins on the P22 Virus-Like Particle Using an External Decoration Protein. ACS Nano, 2015, 9(9), 9134–9147.