Colistin Methanesulfonate (sodium salt)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Colistin methanesulfonate is an inactive prodrug of colistin (also known as polymyxin E), which is an antibiotic with effect against most Gram-negative bacteria, but also causes nephro- and neurotoxicity [1]. Colistin is produced by certain strains of the bacteria Paenibacillus polymyxa, and belongs to the class of polypeptide antibiotics known as polymyxins.
In Vitro: Studying the time-kill kinetics of Colistin methanesulfonate against a type culture of pseudomonas aeruginosa found that 8.0 or 32 mg/l Colistin methanesulfonate began killing at approximately 90 or 30 min, and the mean AUC0-240 values were 186.3±6.0 and 90.4±4.1, respectively [2]. Besides, by investigation among mucoid and nonmucoid strains of Pseudomonas aeruginosa isolated from patients with cystic fibrosis, Colistin methanesulfonate was found to require a concentration of 16 times the MIC to achieve complete killing within 24 h [3].
In Vivo: no data available.
Clinical trial: no data available.
References:
[1] Nation R L, Li J. Colistin in the 21st century.[J]. Current Opinion in Infectious Diseases, 2009, 22(6): 535-543.
[2] Bergen P J, Li J, Rayner C R, et al. Colistin methanesulfonate is an inactive prodrug of colistin against Pseudomonas aeruginosa.[J]. Antimicrobial Agents and Chemotherapy, 2006, 50(6): 1953-1958.
[3] Li J, Turnidge J D, Milne R W, et al. In Vitro Pharmacodynamic Properties of Colistin and Colistin Methanesulfonate against Pseudomonas aeruginosaIsolates from Patients with Cystic Fibrosis[J]. Antimicrobial Agents and Chemotherapy, 2001, 45(3): 781-785.
| Storage | Store at -20°C |
| M.Wt | 1749.8 |
| Cas No. | 8068-28-8 |
| Formula | C58H105N16O28S5·5Na |
| Synonyms | Colimycin M,Polymyxin E sodium methanesulfonate |
| Solubility | ≥53.9 mg/mL in H2O; insoluble in EtOH; insoluble in DMSO |
| Chemical Name | sodium (Z)-N-((6Z,9Z)-7,10-dihydroxy-8-(1-hydroxyethyl)-5-((Z)-oxido(((1Z,4E,7Z,10Z,13Z,16Z,19Z)-2,5,11,14-tetrahydroxy-3-(1-hydroxyethyl)-12,15-diisobutyl-8,17,20-trioxido-6,9,18-tris(2-((sulfomethyl)amino)ethyl)-1,4,7,10,13,16,19-heptaazacyclotricosa-1, |
| SDF | Download SDF |
| Canonical SMILES | CCC(CCCC/C([O-])=N/C(/C(O)=N/C(/C(O)=N/C(/C([O-])=N/C(/C([O-])=N/C(/C([O-])=N/C(/C(O)=N/C(/C(O)=N/C(/C([O-])=N/C(/C(O)=N\1)CCNCS(O)(=O)=O)CCNCS(O)(=O)=O)CC(C)C)CC(C)C)CCNCS(O)(=O)=O)CC/N=C(O)/C1C(O)C)CCNCS(O)(=O)=O)C(O)C)CCNCS(O)(=O)=O)C.[Na+].[Na+].[Na+] |
| Shipping Condition | Ship with blue ice, or upon other requests. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. |
| Cell experiment [1]: | |
|
Cell lines |
P. aeruginosa |
|
Preparation method |
The solubility of this compound in H2O is ≥ 53.9mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
|
Reacting condition |
0.5～1mg/l |
|
Applications |
Colistin methanesulfonate has a MIC range of 0.5 to 1 mg /l. In addition, by investigating among the mucoid and non-mucoid strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients, it was found that colistin methanesulfonate requires 16 times the MIC concentration to completely kill the bacteria within 24 hours. |
|
References: [1]. Bozkurt-Guzel C, Gerceker AA. In vitro pharmacodynamic properties of colistin methanesulfonate and amikacin against Pseudomonas aeruginosa. Indian J Med Microbiol. 2012 Jan-Mar;30(1):34-8. PubMed PMID: 22361758. |
|
Quality Control & MSDS
- View current batch:
-
Potency ≥390 μg/mg
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
