AK Bioluminescence Cytotoxicity Assay Kit
Detects cytotoxicity based on adenylate kinase (AK), high throughput screening (HTS).
Cells treatment with the cytotoxic compound may undergo necrosis, in which they lose membrane integrity. Hence cytotoxicity can be detected by the quantification of plasma membrane damage. Adenylate kinase (AK) is a phosphotransferase enzyme that ubiquitously present in all prokaryotic and eukaryotic cells. When damage of the plasma membrane, AK is rapidly released into the cell culture medium.
The AK Bioluminescence Cytotoxicity Assay Kit provides a simple and fast way for the quantification of cytotoxicity based on the measurement of AK involving two chemical reactions. First, AK that is released from the damaged cells converts ADP to ATP. Second, luciferase catalyzes the formation of light from luciferin and ATP, and the light can be detected using a beta counter or luminometer. The assay is highly sensitive and can be fully automatic for high throughput.
Features & Properties
|Fast and convenient
Simple one-step procedure; takes less than 30 minutes.
The assay is highly sensitive and can be fully automatic for high throughput studies.
|Store at -20°C.
Quality Control & DataSheet
View current batch:
Store at -20°C.
|AK Detection Reagent (Lyophilized)
AK Assay Buffer