AK Bioluminescence Cytotoxicity Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cells treatment with the cytotoxic compound may undergo necrosis, in which they lose membrane integrity. Hence cytotoxicity can be detected by the quantification of plasma membrane damage. Adenylate kinase (AK) is a phosphotransferase enzyme that ubiquitously present in all prokaryotic and eukaryotic cells. When damage of the plasma membrane, AK is rapidly released into the cell culture medium.
The AK Bioluminescence Cytotoxicity Assay Kit provides a simple and fast way for the quantification of cytotoxicity based on the measurement of AK involving two chemical reactions. First, AK that is released from the damaged cells converts ADP to ATP. Second, luciferase catalyzes the formation of light from luciferin and ATP, and the light can be detected using a beta counter or luminometer. The assay is highly sensitive and can be fully automatic for high throughput.
| Features | Fast and convenient Simple one-step procedure; takes less than 30 minutes. The assay is highly sensitive and can be fully automatic for high throughput studies. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
| Kit components | AK Detection Reagent (Lyophilized) AK Assay Buffer |