Caspase-9 Colorimetric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-9 is a member of the caspase family. Caspase-9, as well as caspase-2, -8 and -10, is an initiator caspase. Caspase-9 is involved in the mitochondrial death pathway and is activated during apoptosis. The activation of JNK/SAPK stress signaling pathways induces the release of cytochrome c from mitochondria and activation of apaf-1, which then cleaves caspase-9 into the active form. The active Caspase-9 cleaves and activates Caspase-3 and Caspase-7, which then cleave poly ADP ribose polymerase.
Caspase-9 Colorimetric Assay Kit provides a convenient and simple way for detecting the LEHD-dependent caspase activity. When cleavage of LEHD from the labeled substrate LEHD-p-nitroaniline (LEHD-pNA) by Caspase-9 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-9 activity.
Quality Control & DataSheet
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|Kit components||Cell Lysis Buffer 2X Reaction Buffer LEHD-pNA (4 mM) DTT (1 M) Dilution Buffer|
|Detection method||Absorbance (400 or 405 nm).|
|Sample type||Cell and tissue lysates|
Features & Properties
|Features||Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity.|
|Storage Conditions||Store at -20°C.|