AMI5/Eosin Y disodium salt
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 0.78 and 1.41 μM for Hmt1p and PRMT1, respectively
AMI5/Eosin Y disodium salt is a non-selective protein methyltransferase inhibitor.
Post-translational protein methylation at lysine and arginine residues is related to the gene expression regulation. The enzymatic activities of protein methyltransferases serve to do covalent modifications in the control of gene transcription.
In vitro: AMI5/Eosin Y disodium salt has been identified as a competitive inhibitor of SAM binding and had been shown to inhibit not only PRMTs but also lysine methylation by the Set7 and disruptor of telomeric silencing 1-like (DOT1L) MTases in vitro. Both AMI5/Eosin Y disodium salt and its analog AMI-1 have been used as lead compounds for the development of novel MTase-specific inhibitors. Moreover, it was found that AMI5/Eosin Y disodium salt could inhibit Set7 in vitro and decrease H3K4m1 in vascular endothelial cells. .
In vivo: Currently, there is no animal study reported.
Clinical trial: Up to now, AMI5/Eosin Y disodium salt is still in the preclinical development stage.
 Okabe J,Fernandez AZ,Ziemann M,Keating ST,Balcerczyk A,El-Osta A. Endothelial transcriptome in response to pharmacological methyltransferase inhibition. ChemMedChem.2014 Aug;9(8):1755-62.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥69.2mg/mL in DMSO|
|Chemical Name||sodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
Quality Control & MSDS
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