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Smart-Seq2 Low Input RNA Kit for Sequencing

Catalog No.
K1159
High Sensitivity Low Input cDNA Synthesis & Amplification Kit
Grouped product items
SizePriceStock Qty
12 rxns
$300.00
In stock
24 rxns
$520.00
In stock
96 rxns
$1,768.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

Smart-Seq2 Low Input RNA Kit for Sequencing is a highly sensitive cDNA preparation kit that obtains high-quality full-length cDNA from 10 ng-2 μg total RNA for trace samples RNA-seq analysis. Smart-Seq2 Low Input RNA Kit for Sequencing uses Oligo dT30VN primer as the reverse transcription primer to synthesize cDNA, and relies on the template-switching activity of Reverse Transcriptase to add adapter sequence to the 3'-end reverse transcribed full length cDNA. Through subsequent PCR amplification, to obtain full-length cDNA, effectively avoiding the 3' bias and rRNA contamination. The obtained full-length cDNA product can be used for transcriptome sequencing and gene expression analysis.

Smart-Seq2 Low Input RNA Kit for Sequencing is suitable for the detection of trace samples, so that samples with low abundance and low concentration can also be analyzed by RNA-seq sequencing. In general, one reaction can produce 2-20 ng of cDNA amplification products.

Figure 1. Schematic of the technology in the Smart-Seq2 Low Input RNA Kit for Sequencing

Quality Control

Quality Control & MSDS

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Components and Storage

Components 12 rxns 24 rxns 96 rxns
Box 1 Control Total RNA* (1 μg/μL) 5 μL 5 μL 5 μL
TS Oligo Primer (100 μM) 2.4 μL 4.8 μL 19.2 μL
Box 2 Oligo dT30VN Primer (10 μM) 24 μL 48 μL 192 μL
dNTP Mixture (10 mM) 24 μL 48 μL 192 μL
5X First-Strand Buffer 48 μL 96 μL 384 μL
Reverse Transcriptase (200 U/μL) 12 μL 24 μL 96 μL
RNase Inhibitor, Murine (40 U/μL) 30 μL 60 μL 240 μL
Betaine (5 M) 48 μL 96 μL 384 μL
2X HyPerFUsion® High-Fidelity Master Mix 300 μL 600 μL 1 mL x 3
IS PCR Primer (10 μM) 6 μL 12 μL 48 μL
Elution Buffer 1 mL 1 mL 1 mL x 2
Nuclease-free Water 1 mL 1 mL 1 mL x 2

Box 1: Store at -80°C.

Box 2: Store at -20°C.

*Note: The Control Total RNA is from Hela cell.

Features

Low starting amount of template: Prepare high-quality full-length cDNA with 10 ng-2 μg of total RNA template.

High sensitivity of amplification: Combined with Smart-Seq2 and LNA technology, more genes can be detected.

High success rate of operation: Fewer RNA sample processing steps to avoid the risk of sample loss and increase the success rate of the operation.

Storage

Box 1: Store at -80°C. Box 2: Store at -20°C.