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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hydroxyproline is a common nonproteinogenic amino acid. In mammals, hydroxyproline is found only in elastin and collagen. However, it exists in a variety of other proteins in plants. Hydroxyproline is formed by proline hydroxylase in the peptide chain as a post-translational modification and can not be formed from free proline. A variety of diseases such as neoplastic, renal or bone disease, inflammatory, endocrine and autoimmune disorders affect collagen turnover and cause elevated urine and serum hydroxyproline. Hydroxyproline hydrolysates in tissue can be used for the measurement of the amount of collagen or gelatin present.
The Hydroxyproline Colorimetric Assay Kit provides a sensitive, simple and convenient way for detection of hydroxyproline in tissue or protein/peptide hydrolysates based on colorimetric method. The assay can also be used for measurement of hydroxyproline content in other biological samples such as serum or urine with a prior purification process. The result can be easily qualified using a microtiter plate reader or a spectrophotometer at 560 nm. The assay is useful over the range of 0.1-2 μg.